{"id":366,"date":"2022-03-19T14:28:37","date_gmt":"2022-03-19T07:28:37","guid":{"rendered":"http:\/\/phys-biotech.green.tsu.ru\/?post_type=nproject&p=366"},"modified":"2022-03-19T15:13:09","modified_gmt":"2022-03-19T08:13:09","slug":"microbial-diversity-in-the-deep-subsurfce-resevoir","status":"publish","type":"nproject","link":"https:\/\/phys-biotech.ru\/?nproject=microbial-diversity-in-the-deep-subsurfce-resevoir&lang=en","title":{"rendered":"Microbial diversity in the deep subsurfce resevoir"},"content":{"rendered":"
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Financing Agency: Russian Ministry of Education and Science<\/p>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n

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The deep subsurface biosphere is considered to be a \u201cseed bank\u201d of thermophilic microorganisms. We study microbial diversity in the outflow of a deep (2600 m), artesian, thermal, oil-exploration borehole in Western Siberia.<\/p>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n

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Project Coordinators: Elizaveta Bonch-Osmolovskaya, Olga Karnachuk<\/p>\n

The studied borehole (P3) is in Palaeozoic strata and was never properly sealed following its abandonment. The borehole has been used by the local population as a source of thermal water for the past 40 years. The artesian overflow has a maximum rate of 9.3 m<\/span>3<\/sup>\/day. The artesian character of the borehole is an advantage for microbiological research since it is conceivable that continuous overflow for a period of several decades will have flushed out any possible contamination due to the drilling process.<\/span><\/p>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/section>\n

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PCR-DGGE analysis of 16S rRNA gene fragments, and cultivation in batch culture \/ bioreactor demonstrated that various Firmicutes<\/em>, protoeobacterial Hydrogenophilus<\/em> and methanogenic Archaea<\/em> accounted for a significant component of the bacterial population.<\/p>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/section>\n","protected":false},"excerpt":{"rendered":"

Financing Agency: Russian Ministry of Education and Science The deep subsurface biosphere is considered to be a \u201cseed bank\u201d of thermophilic microorganisms. We study microbial diversity in the outflow of a deep (2600 m), artesian, thermal, oil-exploration borehole in Western … <\/p>\n","protected":false},"author":1,"featured_media":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","format":"standard","meta":{"footnotes":""},"class_list":["post-366","nproject","type-nproject","status-publish","format-standard","hentry"],"_links":{"self":[{"href":"https:\/\/phys-biotech.ru\/index.php?rest_route=\/wp\/v2\/nproject\/366","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/phys-biotech.ru\/index.php?rest_route=\/wp\/v2\/nproject"}],"about":[{"href":"https:\/\/phys-biotech.ru\/index.php?rest_route=\/wp\/v2\/types\/nproject"}],"author":[{"embeddable":true,"href":"https:\/\/phys-biotech.ru\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/phys-biotech.ru\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=366"}],"version-history":[{"count":4,"href":"https:\/\/phys-biotech.ru\/index.php?rest_route=\/wp\/v2\/nproject\/366\/revisions"}],"predecessor-version":[{"id":398,"href":"https:\/\/phys-biotech.ru\/index.php?rest_route=\/wp\/v2\/nproject\/366\/revisions\/398"}],"wp:attachment":[{"href":"https:\/\/phys-biotech.ru\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=366"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}